[Screening, quantification and confirmation of -blockers in urine using gas chromatography-mass spectrometry] Se pu = Chinese journal of chromatography / Zhongguo hua xue hui [Se Pu] Journal article | | Title | [Screening, quantification and confirmation of -blockers in urine using gas chromatography-mass spectrometry] | | Author(s) | Shangguan L, Liu W, Zheng X, Zhang L | | Institution | Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education, Sport Science Research Center, Fuzhou University, Fuzhou 350002, China. shangguan789@163.com | | Source | Se Pu 2008 Jul; 26(4):460-4. | | Abstract | A method was developed to perform the screening, quantification and confirmation of the five beta-blockers, propranolol, carteolol, bisoprolol, esmolol, and sotalol, in human urine using gas chromatography-mass spectrometry (GC-MS). In sample preparation, conjugated and unconjugated beta-blockers in urine were extracted separately, and the extracts were combined. The extracts were derivatized with N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) and N-methyl-bis(trifluoroacetamide) (MBTFA). The optimal conditions of GC-MS were established, and the progresses of screening by selected ion monitoring (SIM) mode and confirmation by full scan (SCAN) mode were completed. At last, the quantification curves of the five beta-blockers in spiked urine were established by SIM mode. The limits of detection were 0.2 -1.0 ng/mL. Overall recoveries were 70.5% - 103.4%, and the relative standard deviations were lower than 15%. In addition, the method was successfully applied to the analysis of the positive urine of propranolol, and the urinary excretion curve was also established accordingly. It is significant to prohibit the abuse of beta-blockers in doping control. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 18959242 |
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